Desired Scaffold Length:
Template material:



Validated primers

SizeForward primer (1 uM)Reverse primer (20 nM)Template
449GTCGTCGTCCCCTCAAACTATTAATGCCGGAGAGGGTAGM13
522GGACGCTATCCAGTCTAAACATGAAAGAGGACAGATGAACGGTGM13
721GTCGTCGTCCCCTCAAACTGCTGAAAAGGTGGCATCAATM13
738CTACCCTCGTTCCGATGCTGTTAATGCCCCCTGCCTATTM13
763GAGCGCAACGCAATTAATGTCAATCATATGTACCCCGGTTGAM13
769GTCGTCGTCCCCTCAAACTGCTGAAAAGGTGGCATCAATM13
771CGCTTTCTTCCCTTCCTTTCTGGCGATTAAGTTGGGTAACGCM13
1,000GTCTCGCTGGTGAAAAGAAAATTAATGCCGGAGAGGGTAGM13
1,018TAATACGACTCACTATAGGTCTCGCTGGTGAAAAGAAAATTAATGCCGGAGAGGGTAGM13
1,021CTCGGTGGCCTCACTGATTATGCTGCAAGGCGATTAAGTTGGM13
1,447TGCCTCAACCTCCTGTCAATAGAGGCATTTTCGAGCCAGTM13
1,521GGGCTTGCTATCCCTGAAAATGACTTGCGGGAGGTTTTGAAGM13
1,616GCGACGATTTACAGAAGCAAAAGGGCGAAAAACCGTCTATM13
2,022GATGAGTGCGGTACTTGGTTTAGCTTTGACGAGCACGTATAACGM13
2,118GCTCCTTCTGGTGGTTTCTTTCTGGCCTTCCTGTAGCCAM13
2,298TACCCTCGTTCCGATGCTGACGACAATAAACAACATGTTCAGCTM13
2,521CACGGTCGGTATTTCAAACCACCTCTTCGCTATTACGCCAGCM13
2,805GTCGTCGTCCCCTCAAACTGTTAATGCCCCCTGCCTATTM13
3,022GCACTGACCCCGTTAAAACTTACAGATTCACCAGTCACACGACCM13
3,281TCTTTGCCTTGCCTGTATGAGCTAACGAGCGTCTTTCCAGM13
3,356GTCTCGCTGGTGAAAAGAAAGTTAATGCCCCCTGCCTATTM13
4,427TGTACTTTGTTTCGCGCTTGAGGGAAGAAAGCGAAAGGAGM13
5,032TGCCTCAACCTCCTGTCAATGCTGAAAAGGTGGCATCAATM13
5,630CTACCCTCGTTCCGATGCTATTAATGCCGGAGAGGGTAGM13
10,000CAGTGCAGTGCTTGATAACAGGGTAGTGCGCGTTTGATTTCCLambda
10,033GCGGTGATACGTGGTGTTTCATAAACGGCAAGATGCGGLambda
11,927CAGTGCAGTGCTTGATAACAGGCCGAAGTCGAAATCAAGCTGLambda
12,000GCGGTGATACGTGGTGTTTCTGGCTTTCAGCTGAATGGTGLambda
15,000GCGGTGATACGTGGTGTTTGCAATTGGCGGTGATGTAAACACLambda